In vivo proteomics approaches for the analysis of bacterial adaptation reactions in host–pathogen settings Chapter uri icon

Overview

MeSH Major

  • Cell Transformation, Viral
  • Oncogenes
  • Receptors, Cell Surface

abstract

  • © Springer Science+Business Media, LLC, part of Springer Nature 2018. Proteome profiling of bacteria internalized by host cells is still a challenging task, due to low amounts of bacterial proteins in host–pathogen settings and the high amounts of contaminating host proteins. Here, we describe a workflow for the enrichment of intracellular bacteria by fluorescence activated cell sorting which in combination with highly sensitive LC-MS/MS allows monitoring of about 1200 proteins from 2 to 4 × 106internalized bacterial cells as starting material.

publication date

  • January 2018

Research

keywords

  • Book Chapter

Identity

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-8695-8_15

Additional Document Info

start page

  • 207

end page

  • 228

volume

  • 1841