In vivo proteomics approaches for the analysis of bacterial adaptation reactions in host–pathogen settings
Cell Transformation, Viral
Receptors, Cell Surface
© Springer Science+Business Media, LLC, part of Springer Nature 2018. Proteome profiling of bacteria internalized by host cells is still a challenging task, due to low amounts of bacterial proteins in host–pathogen settings and the high amounts of contaminating host proteins. Here, we describe a workflow for the enrichment of intracellular bacteria by fluorescence activated cell sorting which in combination with highly sensitive LC-MS/MS allows monitoring of about 1200 proteins from 2 to 4 × 106internalized bacterial cells as starting material.
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