Identification of a Mycothiol-Dependent Nitroreductase from Mycobacterium tuberculosis Academic Article uri icon

Overview

MeSH Major

  • Anti-Bacterial Agents
  • Hydrogen Peroxide
  • Isoniazid
  • Macrophages
  • Microbial Viability
  • Mycobacterium tuberculosis
  • Nitrites

abstract

  • The success of Mycobacterium tuberculosis (Mtb) as a pathogen depends on the redundant and complex mechanisms it has evolved for resisting nitrosative and oxidative stresses inflicted by host immunity. Improving our understanding of these defense pathways can reveal vulnerable points in Mtb pathogenesis. In this study, we combined genetic, structural, computational, biochemical, and biophysical approaches to identify a novel enzyme class represented by Rv2466c. We show that Rv2466c is a mycothiol-dependent nitroreductase of Mtb and can reduce the nitro group of a novel mycobactericidal compound using mycothiol as a cofactor. In addition to its function as a nitroreductase, Rv2466c confers partial protection to menadione stress.

publication date

  • May 11, 2018

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC5952258

Digital Object Identifier (DOI)

  • 10.1021/acsinfecdis.7b00111

PubMed ID

  • 29465985

Additional Document Info

start page

  • 771

end page

  • 787

volume

  • 4

number

  • 5