Sensing Microbial Viability through Bacterial RNA Augments T Follicular Helper Cell and Antibody Responses Academic Article Article uri icon

Overview

MeSH Major

  • Cardiomyopathy, Dilated
  • Heart
  • Muscle Proteins
  • SKP Cullin F-Box Protein Ligases

abstract

  • © 2018 Elsevier Inc. Live vaccines historically afford superior protection, yet the cellular and molecular mechanisms mediating protective immunity remain unclear. Here we found that vaccination of mice with live, but not dead, Gram-negative bacteria heightened follicular T helper cell (Tfh) differentiation, germinal center formation, and protective antibody production through the signaling adaptor TRIF. Complementing the dead vaccine with an innate signature of bacterial viability, bacterial RNA, recapitulated these responses. The interferon (IFN) and inflammasome pathways downstream of TRIF orchestrated Tfh responses extrinsically to B cells and classical dendritic cells. Instead, CX3CR1 + CCR2 – monocytes instructed Tfh differentiation through interleukin-1β (IL-1β), a tightly regulated cytokine secreted upon TRIF-dependent IFN licensing of the inflammasome. Hierarchical production of IFN-β and IL-1β dictated Tfh differentiation and elicited the augmented humoral responses characteristic of live vaccines. These findings identify bacterial RNA, an innate signature of microbial viability, as a trigger for Tfh differentiation and suggest new approaches toward vaccine formulations for coordinating augmented Tfh and B cell responses. Live vaccines typically elicit augmented humoral responses, affording superior protection. Barbet et al. report that innate detection of bacterial RNA, a signature of microbial viability, directs a heightened Tfh cell response. This response is extrinsic to B cells and dendritic cells and involves CX3CR1 + CCR2 – monocyte instruction of Tfh differentiation via TRIF-dependent IFN-β licensing of bacterial RNA-driven inflammasome activation.

publication date

  • January 2018

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/j.immuni.2018.02.015

PubMed ID

  • 29548673

Additional Document Info