Engineered endothelium provides angiogenic and paracrine stimulus to grafted human ovarian tissue. In process uri icon

Overview

abstract

  • Despite major advances in tissue cryopreservation and auto-transplantation, reperfusion ischemia and hypoxia have been reported as major obstacles to successful recovery of the follicular pool within grafted ovarian tissue. We demonstrate a benefit to follicular survival and function in human ovarian tissue that is co-transplanted with exogenous endothelial cells (ExEC). ExECs were capable of forming functionally perfused vessels at the host/graft interface and increased both viability and follicular volume in ExEC-assisted grafts with resumption of antral follicle development in long-term grafts. ExECs that were engineered to constitutively express anti-mullerian hormone (AMH) induced a greater proportion of quiescent primordial follicles than control ExECs, indicating suppression of premature mobilization that has been noted in the context of ovarian tissue transplantation. These findings present a cell-based strategy that combines accelerated perfusion with direct paracrine delivery of a bioactive payload to transplanted ovarian tissue.

publication date

  • August 15, 2017

Research

keywords

  • In press

Identity

Language

  • eng

PubMed Central ID

  • PMC5557862

Digital Object Identifier (DOI)

  • 10.1038/s41598-017-08491-z

PubMed ID

  • 28811567

Additional Document Info

start page

  • 8203

volume

  • 7

number

  • 1