Proteomic and Metabolomic Characterization of a Mammalian Cellular Transition from Quiescence to Proliferation Academic Article uri icon

Overview

MeSH Major

  • Extracellular Signal-Regulated MAP Kinases
  • Indazoles
  • Melanoma
  • Neurofibromin 1
  • Phenformin
  • Piperazines

abstract

  • There exist similarities and differences in metabolism and physiology between normal proliferative cells and tumor cells. Once a cell enters the cell cycle, metabolic machinery is engaged to facilitate various processes. The kinetics and regulation of these metabolic changes have not been properly evaluated. To correlate the orchestration of these processes with the cell cycle, we analyzed the transition from quiescence to proliferation of a non-malignant murine pro-B lymphocyte cell line in response to IL-3. Using multiplex mass-spectrometry-based proteomics, we show that the transition to proliferation shares features generally attributed to cancer cells: upregulation of glycolysis, lipid metabolism, amino-acid synthesis, and nucleotide synthesis and downregulation of oxidative phosphorylation and the urea cycle. Furthermore, metabolomic profiling of this transition reveals similarities to cancer-related metabolic pathways. In particular, we find that methionine is consumed at a higher rate than that of other essential amino acids, with a potential link to maintenance of the epigenome.

publication date

  • July 18, 2017

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC5626450

Digital Object Identifier (DOI)

  • 10.1016/j.celrep.2017.06.074

PubMed ID

  • 28723573

Additional Document Info

start page

  • 721

end page

  • 736

volume

  • 20

number

  • 3