CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells Academic Article uri icon

Overview

MeSH Major

  • Cell Differentiation
  • Endoderm
  • Mesoderm
  • Mouse Embryonic Stem Cells
  • Nodal Protein
  • Phosphoproteins
  • Trans-Activators
  • Tumor Protein p73
  • Tumor Suppressor Protein p53
  • Wnt3 Protein

abstract

  • Human pluripotent stem cells (PSCs) provide an unlimited cell source for cell therapies and disease modeling. Despite their enormous power, technical aspects have hampered reproducibility. Here, we describe a modification of PSC workflows that eliminates a major variable for nearly all PSC experiments: the quality and quantity of the PSC starting material. Most labs continually passage PSCs and use small quantities after expansion, but the "just-in-time" nature of these experiments means that quality control rarely happens before use. Lack of quality control could compromise PSC quality, sterility, and genetic integrity, which creates a variable that might affect results. This method, called CryoPause, banks PSCs as single-use, cryopreserved vials that can be thawed and immediately used in experiments. Each CryoPause bank provides a consistent source of PSCs that can be pre-validated before use to reduce the possibility that high levels of spontaneous differentiation, contamination, or genetic integrity will compromise an experiment.

publication date

  • November 30, 2016

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC5511100

Digital Object Identifier (DOI)

  • 10.1016/j.stemcr.2017.05.010

PubMed ID

  • 28602613

Additional Document Info

start page

  • 355

end page

  • 365