Widespread Mitotic Bookmarking by Histone Marks and Transcription Factors in Pluripotent Stem Cells Academic Article uri icon

Overview

MeSH Major

  • Morphogenesis
  • Proto-Oncogene Proteins B-raf
  • Thyroid Gland
  • Thyroid Hormones
  • Thyroid Neoplasms
  • Twist-Related Protein 2

abstract

  • During mitosis, transcription is halted and many chromatin features are lost, posing a challenge for the continuity of cell identity, particularly in fast cycling stem cells, which constantly balance self-renewal with differentiation. Here we show that, in pluripotent stem cells, certain histone marks and stem cell regulators remain associated with specific genomic regions of mitotic chromatin, a phenomenon known as mitotic bookmarking. Enhancers of stem cell-related genes are bookmarked by both H3K27ac and the master regulators OCT4, SOX2, and KLF4, while promoters of housekeeping genes retain high levels of mitotic H3K27ac in a cell-type invariant manner. Temporal degradation of OCT4 during mitotic exit compromises its ability both to maintain and induce pluripotency, suggesting that its regulatory function partly depends on its bookmarking activity. Together, our data document a widespread yet specific bookmarking by histone modifications and transcription factors promoting faithful and efficient propagation of stemness after cell division.

publication date

  • May 16, 2017

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC5495017

Digital Object Identifier (DOI)

  • 10.1016/j.celrep.2017.04.067

PubMed ID

  • 28514649

Additional Document Info

start page

  • 1283

end page

  • 1293

volume

  • 19

number

  • 7