A Common Origin for B-1a and B-2 Lymphocytes in Clonal Pre- Hematopoietic Stem Cells Academic Article uri icon


MeSH Major

  • Bone Marrow Cells
  • Glioma
  • Myeloid Cells
  • Neovascularization, Pathologic


  • Recent evidence points to the embryonic emergence of some tissue-resident innate immune cells, such as B-1a lymphocytes, prior to and independently of hematopoietic stem cells (HSCs). However, whether the full hematopoietic repertoire of embryonic HSCs initially includes these unique lineages of innate immune cells has been difficult to assess due to lack of clonal assays that identify and assess HSC precursor (pre-HSC) potential. Here, by combining index sorting of single embryonic hemogenic precursors with in┬ávitro HSC maturation and transplantation assays, we analyze emerging pre-HSCs at the single-cell level, revealing their unique stage-specific properties and clonal lineage potential. Remarkably, clonal pre-HSCs detected between E9.5 and E11.5 contribute to the complete B cell repertoire, including B-1a lymphocytes, revealing a previously unappreciated common precursor for all B cell lineages at the pre-HSC stage and a second embryonic origin for B-1a lymphocytes.

publication date

  • June 6, 2017



  • Academic Article



  • eng

PubMed Central ID

  • PMC5469918

Digital Object Identifier (DOI)

  • 10.1016/j.stemcr.2017.04.007

PubMed ID

  • 28479303

Additional Document Info

start page

  • 1563

end page

  • 1572


  • 8


  • 6