Biomarker-based PET imaging of diffuse intrinsic pontine glioma in mouse models Academic Article uri icon

Overview

MeSH Major

  • Brain Stem Neoplasms
  • Glioma
  • Positron-Emission Tomography

abstract

  • Diffuse intrinsic pontine glioma (DIPG) is a childhood brainstem tumor with a universally poor prognosis. Here, we characterize a positron emission tomography (PET) probe for imaging DIPG in vivo In human histological tissues, the probes target, PARP1, was highly expressed in DIPG compared to normal brain. PET imaging allowed for the sensitive detection of DIPG in a genetically engineered mouse model, and probe uptake correlated to histologically determined tumor infiltration. Imaging with the sister fluorescence agent revealed that uptake was confined to proliferating, PARP1-expressing cells. Comparison with other imaging technologies revealed remarkable accuracy of our biomarker approach. We subsequently demonstrated that serial imaging of DIPG in mouse models enables monitoring of tumor growth, as shown in modeling of tumor progression. Overall, this validated method for quantifying DIPG burden would serve useful in monitoring treatment response in early phase clinical trials. Cancer Res; 77(8); 2112-23. ©2017 AACR.

publication date

  • April 15, 2017

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC5392368

Digital Object Identifier (DOI)

  • 10.1158/0008-5472.CAN-16-2850

PubMed ID

  • 28108511

Additional Document Info

start page

  • 2112

end page

  • 2123

volume

  • 77

number

  • 8