A novel technique for generating and observing chemiluminescence in a biological setting Academic Article uri icon


MeSH Major

  • Fluorescent Dyes
  • Nanoparticles
  • Neoplasms
  • Positron-Emission Tomography
  • Zirconium


  • Intraoperative imaging techniques have the potential to make surgical interventions safer and more effective; for these reasons, such techniques are quickly moving into the operating room. Here, we present a new approach that utilizes a technique not yet explored for intraoperative imaging: chemiluminescent imaging. This method employs a ruthenium-based chemiluminescent reporter along with a custom-built nebulizing system to produce ex vivo or in vivo images with high signal-to-noise ratios. The ruthenium-based reporter produces light following exposure to an aqueous oxidizing solution and re-reduction within the surrounding tissue. This method has allowed us to detect reporter concentrations as low as 6.9 pmol/cm(2). In this work, we present a visual guide to our proof-of-concept in vivo studies involving subdermal and intravenous injections in mice. The results suggest that this technology is a promising candidate for further preclinical research and might ultimately become a useful tool in the operating room.

publication date

  • March 9, 2017



  • Academic Article



  • eng

PubMed Central ID

  • PMC5407651

Digital Object Identifier (DOI)

  • 10.3791/54694

PubMed ID

  • 28362395

Additional Document Info


  • 2017


  • 121