Sox17 drives functional engraftment of endothelium converted from non-vascular cells. In process uri icon

Overview

abstract

  • Transplanting vascular endothelial cells (ECs) to support metabolism and express regenerative paracrine factors is a strategy to treat vasculopathies and to promote tissue regeneration. However, transplantation strategies have been challenging to develop, because ECs are difficult to culture and little is known about how to direct them to stably integrate into vasculature. Here we show that only amniotic cells could convert to cells that maintain EC gene expression. Even so, these converted cells perform sub-optimally in transplantation studies. Constitutive Akt signalling increases expression of EC morphogenesis genes, including Sox17, shifts the genomic targeting of Fli1 to favour nearby Sox consensus sites and enhances the vascular function of converted cells. Enforced expression of Sox17 increases expression of morphogenesis genes and promotes integration of transplanted converted cells into injured vessels. Thus, Ets transcription factors specify non-vascular, amniotic cells to EC-like cells, whereas Sox17 expression is required to confer EC function.

publication date

  • January 16, 2017

Research

keywords

  • In press

Identity

Language

  • eng

PubMed Central ID

  • PMC5260855

Digital Object Identifier (DOI)

  • 10.1038/ncomms13963

PubMed ID

  • 28091527

Additional Document Info

start page

  • 13963

volume

  • 8