Integrated genomic DNA/RNA profiling of hematologic malignancies in the clinical setting Academic Article uri icon

Overview

MeSH Major

  • DNA Fingerprinting
  • Gene Expression Profiling
  • Genomics
  • Hematologic Neoplasms

abstract

  • The spectrum of somatic alterations in hematologic malignancies includes substitutions, insertions/deletions (indels), copy number alterations (CNAs), and a wide range of gene fusions; no current clinically available single assay captures the different types of alterations. We developed a novel next-generation sequencing-based assay to identify all classes of genomic alterations using archived formalin-fixed paraffin-embedded blood and bone marrow samples with high accuracy in a clinically relevant time frame, which is performed in our Clinical Laboratory Improvement Amendments-certified College of American Pathologists-accredited laboratory. Targeted capture of DNA/RNA and next-generation sequencing reliably identifies substitutions, indels, CNAs, and gene fusions, with similar accuracy to lower-throughput assays that focus on specific genes and types of genomic alterations. Profiling of 3696 samples identified recurrent somatic alterations that impact diagnosis, prognosis, and therapy selection. This comprehensive genomic profiling approach has proved effective in detecting all types of genomic alterations, including fusion transcripts, which increases the ability to identify clinically relevant genomic alterations with therapeutic relevance.

authors

publication date

  • January 2016

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC4968346

Digital Object Identifier (DOI)

  • 10.1182/blood-2015-08-664649

PubMed ID

  • 26966091

Additional Document Info

start page

  • 3004

end page

  • 14

volume

  • 127

number

  • 24