Regulation of transcriptional elongation in pluripotency and cell differentiation by the PHD-finger protein Phf5a Academic Article Article uri icon

Overview

MeSH Major

  • Calcitriol
  • Parathyroid Hormone
  • Receptors, Calcitriol
  • Transcription, Genetic

abstract

  • © 2016 Macmillan Publishers Limited. part of Springer Nature All rights reserved. Pluripotent embryonic stem cells (ESCs) self-renew or differentiate into all tissues of the developing embryo and cell-specification factors are necessary to balance gene expression. Here we delineate the function of the PHD-finger protein 5a (Phf5a) in ESC self-renewal and ascribe its role in regulating pluripotency, cellular reprogramming and myoblast specification. We demonstrate that Phf5a is essential for maintaining pluripotency, since depleted ESCs exhibit hallmarks of differentiation. Mechanistically, we attribute Phf5a function to the stabilization of the Paf1 transcriptional complex and control of RNA polymerase II elongation on pluripotency loci. Apart from an ESC-specific factor, we demonstrate that Phf5a controls differentiation of adult myoblasts. Our findings suggest a potent mode of regulation by Phf5a in stem cells, which directs their transcriptional programme, ultimately regulating maintenance of pluripotency and cellular reprogramming.

publication date

  • October 27, 2016

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1038/ncb3424

PubMed ID

  • 27749823

Additional Document Info

start page

  • 1127

end page

  • 1138

volume

  • 18

number

  • 11