Cytofluorometric quantification of cell death elicited by NLR proteins Academic Article uri icon

Overview

MeSH Major

  • Apoptosis
  • Morpholines
  • Purines
  • Tumor Suppressor Protein p53

abstract

  • Nucleotide-binding domain and leucine-rich repeat containing (NLR) proteins, also known as NOD-like receptors, are critical components of the molecular machinery that senses intracellular danger signals to initiate an innate immune response against invading pathogens or endogenous sources of hazard. The best characterized effect of NLR signaling is the secretion of various cytokines with immunostimulatory effects, including interleukin (IL)-1β and IL-18. Moreover, at least under specific circumstances, NLRs can promote regulated variants of cell death. Here, we detail two protocols for the cytofluorometric quantification of cell death-associated parameters that can be conveniently employed to assess the lethal activity of specific NLRs or their ligands.

publication date

  • January 2016

Research

keywords

  • Academic Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-3566-6_17

PubMed ID

  • 27221495

Additional Document Info

start page

  • 231

end page

  • 45

volume

  • 1417