Mapping the molecular determinants of BRAF oncogene dependence in human lung cancer Academic Article uri icon

Overview

MeSH Major

  • Autocrine Communication
  • Carcinoma, Non-Small-Cell Lung
  • Drug Resistance, Neoplasm
  • Proto-Oncogene Proteins B-raf
  • Receptor, Epidermal Growth Factor

abstract

  • Oncogenic mutations in the BRAF kinase occur in 6-8% of nonsmall cell lung cancers (NSCLCs), accounting for more than 90,000 deaths annually worldwide. The biological and clinical relevance of these BRAF mutations in NSCLC is incompletely understood. Here we demonstrate that human NSCLC cells with BRAF(V600E), but not other BRAF mutations, initially are sensitive to BRAF-inhibitor treatment. However, these BRAF(V600E) NSCLC cells rapidly acquire resistance to BRAF inhibition through at least one of two discrete molecular mechanisms: (i) loss of full-length BRAF(V600E) coupled with expression of an aberrant form of BRAF(V600E) that retains RAF pathway dependence or (ii) constitutive autocrine EGF receptor (EGFR) signaling driven by c-Jun-mediated EGFR ligand expression. BRAF(V600E) cells with EGFR-driven resistance are characterized by hyperphosphorylated protein kinase AKT, a biomarker we validated in BRAF inhibitor-resistant NSCLC clinical specimens. These data reveal the multifaceted molecular mechanisms by which NSCLCs establish and regulate BRAF oncogene dependence, provide insights into BRAF-EGFR signaling crosstalk, and uncover mechanism-based strategies to optimize clinical responses to BRAF oncogene inhibition.

publication date

  • February 18, 2014

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3932924

Digital Object Identifier (DOI)

  • 10.1073/pnas.1320956111

PubMed ID

  • 24550319

Additional Document Info

start page

  • E748

end page

  • 57

volume

  • 111

number

  • 7