Paper-Based 3D Culture for the Study of Cancer Cells in vitro
Attitude of Health Personnel
Education, Medical, Graduate
© 15CBMS-0001.This paper reports the latest results in the development of stackable and reconfigurable layers of paper as scaffolds for 3D cell culture of cancer cells. Specifically, tissue-like constructs are formed in vitro to recapitulate aspects of the tumor microenvironment, particularly gradients of oxygen. In this work, we demonstrate two new applications of this system: i) a novel 3D migration assay  and ii) a radiosensitivity study. We show that lung cancer cell lines of known differences in their metastatic potential in vivo, respond differently in this system in both their migration behavior and sensitivity to radiation.
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