ZMYND8 Reads the Dual Histone Mark H3K4me1-H3K14ac to Antagonize the Expression of Metastasis-Linked Genes Academic Article uri icon


MeSH Major

  • Cell Movement
  • DNA Methylation
  • Gene Expression Regulation, Neoplastic
  • Histones
  • Lung Neoplasms
  • Prostatic Neoplasms
  • Receptors, Cell Surface


  • Histone acetylation, including acetylated H3K14 (H3K14ac), is generally linked to gene activation. Monomethylated histone H3 lysine 4 (H3K4me1), together with other gene-activating marks, denotes active genes. In contrast to usual gene-activating functions of H3K14ac and H3K4me1, we here show that the dual histone modification mark H3K4me1-H3K14ac is recognized by ZMYND8 (also called RACK7) and can function to counteract gene expression. We identified ZMYND8 as a transcriptional corepressor of the H3K4 demethylase JARID1D. ZMYND8 antagonized the expression of metastasis-linked genes, and its knockdown increased the cellular invasiveness in vitro and in vivo. The plant homeodomain (PHD) and Bromodomain cassette in ZMYND8 mediated the combinatorial recognition of H3K4me1-H3K14ac and H3K4me0-H3K14ac by ZMYND8. These findings uncover an unexpected role for the signature H3K4me1-H3K14ac in attenuating gene expression and reveal a metastasis-suppressive epigenetic mechanism in which ZMYND8's PHD-Bromo cassette couples H3K4me1-H3K14ac with downregulation of metastasis-linked genes.

publication date

  • December 9, 2015



  • Academic Article



  • eng

PubMed Central ID

  • PMC4975651

Digital Object Identifier (DOI)

  • 10.1016/j.molcel.2016.06.035

PubMed ID

  • 27477906

Additional Document Info

start page

  • 470

end page

  • 84