Clinical utility of morphology, immunohistochemistry, flow cytometry, and FISH analysis in monitoring of plasma cell neoplasms in the bone marrow Academic Article uri icon


MeSH Major

  • Adaptor Proteins, Signal Transducing
  • Helicobacter Infections
  • Helicobacter pylori
  • Lymphoma, B-Cell, Marginal Zone
  • Stomach Neoplasms


  • © 2015, Springer-Verlag Berlin Heidelberg.In this study, we examined clinical utility of morphology, immunohistochemistry (IHC), flow cytometric immunophenotyping (FC), and multiple myeloma (MM)-specific fluorescence in situ hybridization (FISH) in evaluation of disease burden in bone marrow (BM) of plasma cell neoplasms (PCNs). Of the 100 consecutive BM samples, 81 demonstrated PCN. CD138 IHC was the most sensitive technique to assess PCN burden compared to aspirate count or flow cytometry with mean values of 30 % (range 3–100), 19 (1–78), and 2.1 (0.01–33.2), respectively. CD138 IHC correlated well with the aspirate count (r2 = 0.5) but poorly with FC (r2 = 0.1). FC demonstrated the highest sensitivity in assessment of clonality 96 % (79/82), while morphology and IHC was positive in 84 % (71/82), FISH in 79 % (59/75) cases. Our results show that CD138 IHC is the most sensitive method for assessment of BM PCN burden whereas FC is the most sensitive method to establish the clonal nature of the PCN.

publication date

  • March 2016



  • Academic Article


Digital Object Identifier (DOI)

  • 10.1007/s12308-015-0264-1

Additional Document Info

start page

  • 9

end page

  • 18


  • 9


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