Principles and Applications of Genomic Diagnostic Techniques
Escherichia coli Infections
Sequence Analysis, DNA
© 2015, 2002 Elsevier Ltd. All rights reserved.Genomic methods such as polymerase chain reaction (PCR) have evolved into faster, more accurate technologies which can not only accurately detect microbial nucleic acids in a wide variety of body samples, but also quantify target genetic material, and in some cases detect and measure microbial gene expression. This has allowed clinicians to better characterize microbial behaviour and interactions during states of health and disease, and to identify unique phenotypic and genotypic markers for fingerprinting in outbreaks or epidemics. As these new technologies become available, a judicious and careful assessment of these approaches will be necessary to better understand their clinical diagnostic value. Although these tests have greatly empowered clinicians and microbiologists to diagnose infections in a manner never before possible, correct interpretation of the results has never been more important. In this chapter we review current genomic technologies available, with attention to clinical applications, limitations, and interpretation in the medical setting.
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