Detection and delineation of oral cancer with a PARP1 targeted optical imaging agent Academic Article uri icon

Overview

MeSH Major

  • Early Detection of Cancer
  • Mouth Neoplasms
  • Poly (ADP-Ribose) Polymerase-1

abstract

  • Earlier and more accurate detection of oral squamous cell carcinoma (OSCC) is essential to improve the prognosis of patients and to reduce the morbidity of surgical therapy. Here, we demonstrate that the nuclear enzyme Poly(ADP-ribose)Polymerase 1 (PARP1) is a promising target for optical imaging of OSCC with the fluorescent dye PARPi-FL. In patient-derived OSCC specimens, PARP1 expression was increased 7.8 ± 2.6-fold when compared to normal tissue. Intravenous injection of PARPi-FL allowed for high contrast in vivo imaging of human OSCC models in mice with a surgical fluorescence stereoscope and high-resolution imaging systems. The emitted signal was specific for PARP1 expression and, most importantly, PARPi-FL can be used as a topical imaging agent, spatially resolving the orthotopic tongue tumors in vivo. Collectively, our results suggest that PARP1 imaging with PARPi-FL can enhance the detection of oral cancer, serve as a screening tool and help to guide surgical resections.

publication date

  • February 22, 2016

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC4761964

Digital Object Identifier (DOI)

  • 10.1038/srep21371

PubMed ID

  • 26900125

Additional Document Info

start page

  • 21371

volume

  • 6