Use of tethered enzymes as a platform technology for rapid analyte detection Academic Article uri icon

Overview

MeSH Major

  • Aging
  • Biological Assay
  • Enzymes, Immobilized
  • Infarction, Middle Cerebral Artery
  • Phosphopyruvate Hydratase
  • Stroke

abstract

  • Oriented enzyme immobilization conferred more efficient coupled activity, and thus higher assay sensitivity, than non-tethered enzymes. Together, our findings provide proof of concept for using oriented immobilization of active enzymes on NPs as the basis for a highly rapid and sensitive biomarker detection platform. This addresses a key challenge in developing a PoCT platform for time sensitive and difficult to diagnose pathologies.

publication date

  • November 2015

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC4659663

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0142326

PubMed ID

  • 26605916

Additional Document Info

start page

  • e0142326

volume

  • 10

number

  • 11