Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond timescale. Academic Article uri icon

Overview

MeSH

  • Algorithms
  • Bacteria
  • Fluorescence Resonance Energy Transfer
  • Humans
  • Time Factors

MeSH Major

  • Image Processing, Computer-Assisted
  • Microscopy, Fluorescence
  • Molecular Imaging
  • RNA, Transfer
  • Ribosomes

abstract

  • Single-molecule fluorescence microscopy is uniquely suited for detecting transient molecular recognition events, yet achieving the time resolution and statistics needed to realize this potential has proven challenging. Here we present a single-molecule imaging and analysis platform using scientific complementary metal-oxide semiconductor (sCMOS) detectors that enables imaging of 15,000 individual molecules simultaneously at millisecond rates. This system enabled the detection of previously obscured processes relevant to the fidelity mechanism in protein synthesis.

publication date

  • April 2016

has subject area

  • Algorithms
  • Bacteria
  • Fluorescence Resonance Energy Transfer
  • Humans
  • Image Processing, Computer-Assisted
  • Microscopy, Fluorescence
  • Molecular Imaging
  • RNA, Transfer
  • Ribosomes
  • Time Factors

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC4814340

Digital Object Identifier (DOI)

  • 10.1038/nmeth.3769

PubMed ID

  • 26878382

Additional Document Info

start page

  • 341

end page

  • 344

volume

  • 13

number

  • 4