Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond timescale Academic Article uri icon


MeSH Major

  • Image Processing, Computer-Assisted
  • Microscopy, Fluorescence
  • Molecular Imaging
  • RNA, Transfer
  • Ribosomes


  • Single-molecule fluorescence microscopy is uniquely suited for detecting transient molecular recognition events, yet achieving the time resolution and statistics needed to realize this potential has proven challenging. Here we present a single-molecule imaging and analysis platform using scientific complementary metal-oxide semiconductor (sCMOS) detectors that enables imaging of 15,000 individual molecules simultaneously at millisecond rates. This system enabled the detection of previously obscured processes relevant to the fidelity mechanism in protein synthesis.

publication date

  • February 15, 2016



  • Academic Article



  • eng

PubMed Central ID

  • PMC4814340

Digital Object Identifier (DOI)

  • 10.1038/nmeth.3769

PubMed ID

  • 26878382

Additional Document Info