FNA needle rinses preserved in Cytolyt are acceptable specimen type for mutation testing of thyroid nodules Academic Article uri icon


MeSH Major

  • Papanicolaou Test
  • Papillomavirus Infections
  • Uterine Cervical Neoplasms
  • Vaginal Smears


  • © 2015 American Society of Cytopathology. Introduction: This study investigated the application of molecular testing to residual thyroid fine-needle aspiration material from needle rinses collected in Cytolyt. Materials and methods: Two thyroid needle rinses from 135 patients were collected in Cytolyt during routine diagnostic workup in our institution. Molecular testing was performed to detect 14 genetic alterations in BRAF, K-, H-, N-RAS genes as well as RET/PTC1, RET/PTC3, and PAX8/PPARγ and verified by next generation sequencing and correlated with cytologic diagnoses. Results: Molecular testing revealed a total of 17 mutations across specimens with benign nodule (n = 5; HRAS, NRAS), Hürthle cell neoplasm (n = 2; BRAF, HRAS) and Papillary thyroid carcinoma (n = 10, 9. BRAF, 1 KRAS) cytology. No RNA gene rearrangements were detected. Conclusions: Mutations and translocations associated with thyroid cancer can be detected in thyroid fine-needle aspiration needle rinses preserved in Cytolyt specimens collected during routine patient management, which are typically discarded when a diagnosis is attained.

publication date

  • May 2015



  • Academic Article


Digital Object Identifier (DOI)

  • 10.1016/j.jasc.2015.01.001

Additional Document Info

start page

  • 128

end page

  • 135


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