Ribosomal RNA gene sequencing for early diagnosis of Blastomyces dermatitidis infection Academic Article uri icon


MeSH Major

  • Blastomyces
  • Blastomycosis
  • Genes, rRNA


  • Prompt detection and identification of fungal pathogens at the genus and species level is critical in order to provide timely antifungal therapy. Here, we highlight the vital role of molecular diagnostics in achieving a fast and definitive diagnosis of disseminated blastomycosis in a diabetic patient presenting as a brain mass initially thought to be tumoral in nature. A broad-range PCR amplification and sequencing of the fungal ribosomal RNA genes on brain biopsy tissue obtained during elective craniotomy revealed a final microbial identification of Ajellomyces dermatitidis (telemorph of Blastomyces dermatitidis).

publication date

  • August 2015



  • Academic Article



  • eng

Digital Object Identifier (DOI)

  • 10.1016/j.ijid.2015.06.017

PubMed ID

  • 26129971

Additional Document Info

start page

  • 122

end page

  • 4


  • 37