Assessment of reproducibility of matrix-assisted laser desorption ionization - Time of flight mass spectrometry for bacterial and yeast identification Academic Article uri icon

Overview

MeSH Major

  • Bacteria
  • Microbiological Techniques
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Yeasts

abstract

  • Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has revolutionized the identification of clinical bacterial and yeast isolates. However, data describing the reproducibility of MALDI-TOF MS for microbial identification are scarce. In this study, we show that MALDI-TOF MS-based microbial identification is highly reproducible and can tolerate numerous variables, including differences in testing environments, instruments, operators, reagent lots, and sample positioning patterns. Finally, we reveal that samples of bacterial and yeast isolates prepared for MALDI-TOF MS identification can be repeatedly analyzed without compromising organism identification.

publication date

  • January 2015

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC4473194

Digital Object Identifier (DOI)

  • 10.1128/JCM.00187-15

PubMed ID

  • 25926486

Additional Document Info

start page

  • 2349

end page

  • 52

volume

  • 53

number

  • 7