A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation of Behavior Academic Article uri icon

Overview

MeSH Major

  • Behavior, Animal
  • Diterpenes
  • Neurons
  • Receptors, Opioid, kappa
  • Signal Transduction

abstract

  • DREADDs are chemogenetic tools widely used to remotely control cellular signaling, neuronal activity, and behavior. Here we used a structure-based approach to develop a new Gi-coupled DREADD using the kappa-opioid receptor as a template (KORD) that is activated by the pharmacologically inert ligand salvinorin B (SALB). Activation of virally expressed KORD in several neuronal contexts robustly attenuated neuronal activity and modified behaviors. Additionally, co-expression of the KORD and the Gq-coupled M3-DREADD within the same neuronal population facilitated the sequential and bidirectional remote control of behavior. The availability of DREADDs activated by different ligands provides enhanced opportunities for investigating diverse physiological systems using multiplexed chemogenetic actuators.

publication date

  • January 2015

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC4441592

Digital Object Identifier (DOI)

  • 10.1016/j.neuron.2015.03.065

PubMed ID

  • 25937170

Additional Document Info

start page

  • 936

end page

  • 46

volume

  • 86

number

  • 4