Persistence of smoking-induced dysregulation of miRNA expression in the small airway epithelium despite smoking cessation. Academic Article uri icon

Overview

MeSH

  • Adult
  • Bronchoscopy
  • Cell Differentiation
  • Cluster Analysis
  • Cotinine
  • Down-Regulation
  • Female
  • Humans
  • Male
  • Middle Aged
  • Nicotine
  • Smoking Cessation
  • Up-Regulation
  • Wnt Signaling Pathway

MeSH Major

  • Epithelium
  • MicroRNAs
  • Respiratory Mucosa
  • Smoking

abstract

  • Even after quitting smoking, the risk of the development of chronic obstructive pulmonary disease (COPD) and lung cancer remains significantly higher compared to healthy nonsmokers. Based on the knowledge that COPD and most lung cancers start in the small airway epithelium (SAE), we hypothesized that smoking modulates miRNA expression in the SAE linked to the pathogenesis of smoking-induced airway disease, and that some of these changes persist after smoking cessation. SAE was collected from 10th to 12th order bronchi using fiberoptic bronchoscopy. Affymetrix miRNA 2.0 arrays were used to assess miRNA expression in the SAE from 9 healthy nonsmokers and 10 healthy smokers, before and after they quit smoking for 3 months. Smoking status was determined by urine nicotine and cotinine measurement. There were significant differences in the expression of 34 miRNAs between healthy smokers and healthy nonsmokers (p<0.01, fold-change >1.5), with functions associated with lung development, airway epithelium differentiation, inflammation and cancer. After quitting smoking for 3 months, 12 out of the 34 miRNAs did not return to normal levels, with Wnt/β-catenin signaling pathway being the top identified enriched pathway of the target genes of the persistent dysregulated miRNAs. In the context that many of these persistent smoking-dependent miRNAs are associated with differentiation, inflammatory diseases or lung cancer, it is likely that persistent smoking-related changes in SAE miRNAs play a role in the subsequent development of these disorders.

publication date

  • 2015

has subject area

  • Adult
  • Bronchoscopy
  • Cell Differentiation
  • Cluster Analysis
  • Cotinine
  • Down-Regulation
  • Epithelium
  • Female
  • Humans
  • Male
  • MicroRNAs
  • Middle Aged
  • Nicotine
  • Respiratory Mucosa
  • Smoking
  • Smoking Cessation
  • Up-Regulation
  • Wnt Signaling Pathway

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC4401720

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0120824

PubMed ID

  • 25886353

Additional Document Info

start page

  • e0120824

volume

  • 10

number

  • 4