Protein expression profiles characterize distinct features of mouse cerebral cortices at different developmental stages Academic Article uri icon


MeSH Major

  • Cerebral Cortex
  • Nerve Tissue Proteins


  • The proper development of the mammalian cerebral cortex requires precise protein synthesis and accurate regulation of protein expression levels. To reveal signatures of protein expression in developing mouse cortices, we here generate proteomic profiles of cortices at embryonic and postnatal stages using tandem mass spectrometry (MS/MS). We found that protein expression profiles are mostly consistent with biological features of the developing cortex. Gene Ontology (GO) and KEGG pathway analyses demonstrate conserved molecules that maintain cortical development such as proteins involved in metabolism. GO and KEGG pathway analyses further identify differentially expressed proteins that function at specific stages, for example proteins regulating the cell cycle in the embryonic cortex, and proteins controlling axon guidance in the postnatal cortex, suggesting that distinct protein expression profiles determine biological events in the developing cortex. Furthermore, the STRING network analysis has revealed that many proteins control a single biological event, such as the cell cycle regulation, through cohesive interactions, indicating a complex network regulation in the cortex. Our study has identified protein networks that control the cortical development and has provided a protein reference for further investigation of protein interactions in the cortex.

publication date

  • April 27, 2015



  • Academic Article



  • eng

PubMed Central ID

  • PMC4411115

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0125608

PubMed ID

  • 25915664

Additional Document Info

start page

  • e0125608


  • 10


  • 4