Unsaturated fatty acids induce non-canonical autophagy Academic Article uri icon


MeSH Major

  • Autophagy
  • Fatty Acids, Unsaturated


  • To obtain mechanistic insights into the cross talk between lipolysis and autophagy, two key metabolic responses to starvation, we screened the autophagy-inducing potential of a panel of fatty acids in human cancer cells. Both saturated and unsaturated fatty acids such as palmitate and oleate, respectively, triggered autophagy, but the underlying molecular mechanisms differed. Oleate, but not palmitate, stimulated an autophagic response that required an intact Golgi apparatus. Conversely, autophagy triggered by palmitate, but not oleate, required AMPK, PKR and JNK1 and involved the activation of the BECN1/PIK3C3 lipid kinase complex. Accordingly, the downregulation of BECN1 and PIK3C3 abolished palmitate-induced, but not oleate-induced, autophagy in human cancer cells. Moreover, Becn1(+/-) mice as well as yeast cells and nematodes lacking the ortholog of human BECN1 mounted an autophagic response to oleate, but not palmitate. Thus, unsaturated fatty acids induce a non-canonical, phylogenetically conserved, autophagic response that in mammalian cells relies on the Golgi apparatus.


publication date

  • January 2015



  • Academic Article



  • eng

PubMed Central ID

  • PMC4406650

Digital Object Identifier (DOI)

  • 10.15252/embj.201489363

PubMed ID

  • 25586377

Additional Document Info

start page

  • 1025

end page

  • 41


  • 34


  • 8