Differentiation of human pluripotent stem cells to cells similar to cord-blood endothelial colony-forming cells. Academic Article uri icon

Overview

MeSH

  • Animals
  • Cell Proliferation
  • Fetal Blood
  • Humans
  • Mice
  • Neuropilin-1
  • Stem Cells
  • Vascular Endothelial Growth Factor A

MeSH Major

  • Cell Differentiation
  • Embryonic Stem Cells
  • Endothelial Cells
  • Pluripotent Stem Cells

abstract

  • The ability to differentiate human pluripotent stem cells into endothelial cells with properties of cord-blood endothelial colony-forming cells (CB-ECFCs) may enable the derivation of clinically relevant numbers of highly proliferative blood vessel-forming cells to restore endothelial function in patients with vascular disease. We describe a protocol to convert human induced pluripotent stem cells (hiPSCs) or embryonic stem cells (hESCs) into cells similar to CB-ECFCs at an efficiency of >10(8) ECFCs produced from each starting pluripotent stem cell. The CB-ECFC-like cells display a stable endothelial phenotype with high clonal proliferative potential and the capacity to form human vessels in mice and to repair the ischemic mouse retina and limb, and they lack teratoma formation potential. We identify Neuropilin-1 (NRP-1)-mediated activation of KDR signaling through VEGF165 as a critical mechanism for the emergence and maintenance of CB-ECFC-like cells.

publication date

  • November 2014

has subject area

  • Animals
  • Cell Differentiation
  • Cell Proliferation
  • Embryonic Stem Cells
  • Endothelial Cells
  • Fetal Blood
  • Humans
  • Mice
  • Neuropilin-1
  • Pluripotent Stem Cells
  • Stem Cells
  • Vascular Endothelial Growth Factor A

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC4318247

Digital Object Identifier (DOI)

  • 10.1038/nbt.3048

PubMed ID

  • 25306246

Additional Document Info

start page

  • 1151

end page

  • 1157

volume

  • 32

number

  • 11