A multi-stress model for high throughput screening against non-replicating Mycobacterium tuberculosis Academic Article uri icon

Overview

MeSH Major

  • Antitubercular Agents
  • High-Throughput Screening Assays
  • Microbial Sensitivity Tests
  • Mycobacterium tuberculosis
  • Stress, Physiological

abstract

  • Models of non-replication help us understand the biology of persistent Mycobacterium tuberculosis. High throughput screening (HTS) against non-replicating M. tuberculosis may lead to identification of tool compounds that affect pathways on which bacterial survival depends in such states, and to development of drugs that can overcome phenotypic tolerance to conventional antimycobacterial agents, which are mostly active against replicating M. tuberculosis. We describe a multi-stress model of non-replication that mimics some of the microenvironmental conditions that M. tuberculosis faces in the host as adapted for HTS. The model includes acidic pH, mild hypoxia, a flux of nitric oxide and other reactive nitrogen intermediates arising from nitrite at low pH, and low concentrations of a fatty acid (butyrate) as a carbon source.

publication date

  • January 2015

Research

keywords

  • Academic Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-2450-9_18

PubMed ID

  • 25779324

Additional Document Info

start page

  • 293

end page

  • 315

volume

  • 1285