Histone variant H2A.X deposition pattern serves as a functional epigenetic mark for distinguishing the developmental potentials of iPSCs. Academic Article uri icon

Overview

MeSH

  • Animals
  • Base Sequence
  • Cell Differentiation
  • Cell Line
  • Cell Lineage
  • Clone Cells
  • Embryonic Stem Cells
  • Female
  • Gene Expression Regulation, Developmental
  • Mice, Inbred ICR
  • Models, Biological
  • Molecular Sequence Data
  • Up-Regulation

MeSH Major

  • Epigenesis, Genetic
  • Histones
  • Induced Pluripotent Stem Cells

abstract

  • For future application of induced pluripotent stem cell (iPSC) technology, the ability to assess the overall quality of iPSC clones will be an important issue. Here we show that the histone variant H2A.X is a functional marker that can distinguish the developmental potentials of mouse iPSC lines. We found that H2A.X is specifically targeted to and negatively regulates extraembryonic lineage gene expression in embryonic stem cells (ESCs) and prevents trophectoderm lineage differentiation. ESC-specific H2A.X deposition patterns are faithfully recapitulated in iPSCs that support the development of "all-iPS" animals via tetraploid complementation, the most stringent test available of iPSC quality. In contrast, iPSCs that fail to support all-iPS embryonic development show aberrant H2A.X deposition, upregulation of extraembryonic lineage genes, and a predisposition to extraembryonic differentiation. Thus, our work has highlighted an epigenetic mechanism for maintaining cell lineage commitment in ESCs and iPSCs that can be used to distinguish the quality of iPSC lines. Copyright © 2014 Elsevier Inc. All rights reserved.

publication date

  • September 4, 2014

has subject area

  • Animals
  • Base Sequence
  • Cell Differentiation
  • Cell Line
  • Cell Lineage
  • Clone Cells
  • Embryonic Stem Cells
  • Epigenesis, Genetic
  • Female
  • Gene Expression Regulation, Developmental
  • Histones
  • Induced Pluripotent Stem Cells
  • Mice, Inbred ICR
  • Models, Biological
  • Molecular Sequence Data
  • Up-Regulation

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1016/j.stem.2014.06.004

PubMed ID

  • 25192463

Additional Document Info

start page

  • 281

end page

  • 294

volume

  • 15

number

  • 3