Quantitative multiplexed detection of common pulmonary fungal pathogens by labeled primer polymerase chain reaction
Lung Diseases, Fungal
Multiplex Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Labeled primer polymerase chain reaction permits rapid, quantitative detection and differentiation of common agents of invasive fungal infection. The assay described herein shows promise for clinical implementation that may have a significant effect on the rapid diagnosis and treatment of patients' severe infections caused by these pulmonary fungal pathogens.