ID1 is a functional marker for intestinal stem and progenitor cells required for normal response to injury Academic Article uri icon


MeSH Major

  • Biomarkers
  • Inhibitor of Differentiation Protein 1
  • Intestines
  • Multipotent Stem Cells


  • LGR5 and BMI1 mark intestinal stem cells in crypt base columnar cells and +4 position cells, respectively, but characterization of functional markers in these cell populations is limited. ID1 maintains the stem cell potential of embryonic, neural, and long-term repopulating hematopoietic stem cells. Here, we show in both human and mouse intestine that ID1 is expressed in cycling columnar cells, +4 position cells, and transit-amplifying cells in the crypt. Lineage tracing revealed ID1+ cells to be self-renewing, multipotent stem/progenitor cells that are responsible for the long-term renewal of the intestinal epithelium. Single ID1+ cells can generate long-lived organoids resembling mature intestinal epithelium. Complete knockout of Id1 or selective deletion of Id1 in intestinal epithelium or in LGR5+ stem cells sensitizes mice to chemical-induced colon injury. These experiments identify ID1 as a marker for intestinal stem/progenitor cells and demonstrate a role for ID1 in maintaining the potential for repair in response to colonic injury.

publication date

  • January 2014



  • Academic Article



  • eng

PubMed Central ID

  • PMC4235234

Digital Object Identifier (DOI)

  • 10.1016/j.stemcr.2014.09.012

PubMed ID

  • 25418719

Additional Document Info

start page

  • 716

end page

  • 24


  • 3


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