CAMP-responsive element-binding protein (CREB) and cAMP co-regulate activator protein 1 (AP1)-dependent regeneration-associated gene expression and neurite growth Academic Article uri icon

Overview

MeSH Major

  • Cyclic AMP
  • Cyclic AMP Response Element-Binding Protein
  • Gene Expression
  • Neurites
  • Transcription Factor AP-1

abstract

  • To regenerate damaged axons, neurons must express a cassette of regeneration-associated genes (RAGs) that increases intrinsic growth capacity and confers resistance to extrinsic inhibitory cues. Here we show that dibutyrl-cAMP or forskolin combined with constitutive-active CREB are superior to either agent alone in driving neurite growth on permissive and inhibitory substrates. Of the RAGs examined, only arginase 1 (Arg1) expression correlated with the increased neurite growth induced by the cAMP/CREB combination, both of which were AP1-dependent. This suggests that cAMP-induced AP1 activity is necessary and interacts with CREB to drive expression of RAGs relevant for regeneration and demonstrates that combining a small molecule (cAMP) with an activated transcription factor (CREB) stimulates the gene expression necessary to enhance axonal regeneration.

publication date

  • November 21, 2014

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC4239638

Digital Object Identifier (DOI)

  • 10.1074/jbc.M114.582460

PubMed ID

  • 25296755

Additional Document Info

start page

  • 32914

end page

  • 25

volume

  • 289

number

  • 47