Maternal immunization with chimpanzee adenovirus expressing RSV fusion protein protects against neonatal RSV pulmonary infection. Academic Article uri icon

Overview

MeSH

  • Adenoviridae
  • Administration, Intranasal
  • Animals
  • Animals, Newborn
  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Cross Reactions
  • Female
  • Immunity, Humoral
  • Immunity, Mucosal
  • Mice, Inbred BALB C
  • Respiratory Syncytial Viruses

MeSH Major

  • Immunity, Maternally-Acquired
  • Respiratory Syncytial Virus Infections
  • Respiratory Syncytial Virus Vaccines
  • Viral Fusion Proteins

abstract

  • Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract disease with high morbidity and mortality in young infants and children. Despite numerous efforts, a licensed vaccine against RSV remains elusive. Since young infants form the primary target group of RSV disease, maternal immunization to boost the protection in neonates is an attractive strategy. In this study we tested the efficacy of maternal immunization with a chimpanzee adenovirus expressing codon-optimized RSV fusion protein (AdC7-Fsyn) to protect infants against RSV infection. Single intranasal immunization of mice by AdC7-Fsyn induced robust anti-RSV systemic and mucosal immunity that protected against RSV without causing vaccine-enhanced RSV disease. RSV humoral immunity was transferred to pups born to immunized mothers that provided protection against RSV. Immunization with AdC7-Fsyn was effective even in the presence of Ad5 preimmunity. The maternally derived immunity was durable with the half-life of 14.63 days that reduced the viral replication up to 15 weeks of age. Notably, the passively immunized mice could be actively re-immunized with AdC7-Fsyn to boost and extend the protection. This substantiates maternal immunization with an AdC7-based vaccine expressing RSV F as feasible approach to protect against RSV early in life. Copyright © 2014 Elsevier Ltd. All rights reserved.

publication date

  • September 29, 2014

has subject area

  • Adenoviridae
  • Administration, Intranasal
  • Animals
  • Animals, Newborn
  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Cross Reactions
  • Female
  • Immunity, Humoral
  • Immunity, Maternally-Acquired
  • Immunity, Mucosal
  • Mice, Inbred BALB C
  • Respiratory Syncytial Virus Infections
  • Respiratory Syncytial Virus Vaccines
  • Respiratory Syncytial Viruses
  • Viral Fusion Proteins

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC4713013

Digital Object Identifier (DOI)

  • 10.1016/j.vaccine.2014.08.049

PubMed ID

  • 25171847

Additional Document Info

start page

  • 5761

end page

  • 5768

volume

  • 32

number

  • 43