Cecal ligation and puncture-induced sepsis as a model to study autophagy in mice. Review uri icon

Overview

MeSH

  • Animals
  • Ligation
  • Mice
  • Mice, Transgenic

MeSH Major

  • Autophagy
  • Cecum
  • Disease Models, Animal
  • Sepsis

abstract

  • Experimental sepsis can be induced in mice using the cecal ligation and puncture (CLP) method, which causes polymicrobial sepsis. Here, a protocol is provided to induce sepsis of varying severity in mice using the CLP technique. Autophagy is a fundamental tissue response to stress and pathogen invasion. Two current protocols to assess autophagy in vivo in the context of experimental sepsis are also presented here. (I) Transgenic mice expressing green fluorescence protein (GFP)-LC3 fusion protein are subjected to CLP. Localized enhancement of GFP signal (puncta), as assayed either by immunohistochemical or confocal assays, can be used to detect enhanced autophagosome formation and, thus, altered activation of the autophagy pathway. (II) Enhanced autophagic vacuole (autophagosome) formation per unit tissue area (as a marker of autophagy stimulation) can be quantified using electron microscopy. The study of autophagic responses to sepsis is a critical component of understanding the mechanisms by which tissues respond to infection. Research findings in this area may ultimately contribute towards understanding the pathogenesis of sepsis, which represents a major problem in critical care medicine.

publication date

  • 2014

has subject area

  • Animals
  • Autophagy
  • Cecum
  • Disease Models, Animal
  • Ligation
  • Mice
  • Mice, Transgenic
  • Sepsis

Research

keywords

  • Journal Article
  • Review

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.3791/51066

PubMed ID

  • 24561344

Additional Document Info

start page

  • e51066

number

  • 84