Spexin is a novel human peptide that reduces adipocyte uptake of long chain fatty acids and causes weight loss in rodents with diet-induced obesity. Academic Article uri icon

Overview

MeSH

  • Animals
  • Body Weight
  • Down-Regulation
  • Eating
  • Feeding Behavior
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Obese
  • Oleic Acid
  • Protein Array Analysis
  • Rats
  • Rats, Wistar

MeSH Major

  • Adipocytes
  • Energy Intake
  • Fatty Acids
  • Leptin
  • Obesity
  • Peptide Hormones
  • Weight Loss

abstract

  • Microarray studies identified Ch12:orf39 (Spexin) as the most down-regulated gene in obese human fat. Therefore, we examined its role in obesity pathogenesis. Spexin effects on food intake, meal patterns, body weight, respiratory exchange ratio (RER), and locomotor activity were monitored electronically in C57BL/6J mice or Wistar rats with diet-induced obesity (DIO). Its effects on adipocyte [(3)H]-oleate uptake were determined. In humans, Spexin gene expression was down-regulated 14.9-fold in obese omental and subcutaneous fat. Circulating Spexin changed in parallel, correlating (r = -0.797) with Leptin. In rats, Spexin (35 µg/kg/day SC) reduced caloric intake ∼32% with corresponding weight loss. Meal patterns were unaffected. In mice, Spexin (25 µg/kg/day IP) significantly reduced the RER at night, and increased locomotion. Spexin incubation in vitro significantly inhibited facilitated fatty acid (FA) uptake into DIO mouse adipocytes. Conditioned taste aversion testing (70 µg/kg/day IP) demonstrated no aversive Spexin effects. Spexin gene expression is markedly down-regulated in obese human fat. The peptide produces weight loss in DIO rodents. Its effects on appetite and energy regulation are presumably central; those on adipocyte FA uptake appear direct and peripheral. Spexin is a novel hormone involved in weight regulation, with potential for obesity therapy. Copyright © 2014 The Obesity Society.

publication date

  • July 2014

has subject area

  • Adipocytes
  • Animals
  • Body Weight
  • Down-Regulation
  • Eating
  • Energy Intake
  • Fatty Acids
  • Feeding Behavior
  • Humans
  • Leptin
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Obese
  • Obesity
  • Oleic Acid
  • Peptide Hormones
  • Protein Array Analysis
  • Rats
  • Rats, Wistar
  • Weight Loss

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC4077920

Digital Object Identifier (DOI)

  • 10.1002/oby.20725

PubMed ID

  • 24550067

Additional Document Info

start page

  • 1643

end page

  • 1652

volume

  • 22

number

  • 7