High efficiency capture of circulating tumor cells from patient blood using geometrically-enhanced differential immunocapture
Microfluidic Analytical Techniques
Neoplastic Cells, Circulating
Here we report the capture of circulating prostate tumor cells (PCTCs) from whole peripheral blood samples of patients with castrate-resistant prostate cancer. Using a microfluidic device geometry that maximizes streamline distortion and target cell-wall collisions, we have been able to capture cells with higher efficiencies and purities that exceed previously reported values. Potential applications for this device include capture of PCTCs to study the molecular mechanisms of clinical drug resistance, profiling of individual patients' CTCs in order to generate personalized chemotherapeutics, as well as real-time monitoring of chemotherapeutic efficacy using a non-invasive blood-based assay. © 2009 CBMS.
Additional Document Info