Microfluidic three-dimensional cellular patterning for evaluation of cell-cell interaction dynamics
Cell Culture Techniques
Here we report the application of a microfluidic 3D cell pattening method to evaluation of cell-cell interaction dynamics. We show that for some cell combinations, initial cell positioning affects subsequent spheroid formation resulting in two juxtaposed non-concentric "Janus" spheroids of different cell types, rather than homogeneous mixtures or layered shell structures. Janus spheroids of mouse embryonic stem cells and hepatocarcinoma cells resulted in spatially patterned differentiation of mES cells, demonstrating that cellular differentiation can be controlled by intercellular positioning. This cellular patterning method opens new possibilities for understanding and manipulating interactions between different cell types in 3D. © 2009 CBMS.
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