Stable 293 T and CHO cell lines expressing cleaved, stable HIV-1 envelope glycoprotein trimers for structural and vaccine studies Academic Article uri icon

Overview

MeSH Major

  • Antigens, Viral
  • Gene Expression
  • Glycoproteins
  • HIV-1
  • Vaccines
  • env Gene Products, Human Immunodeficiency Virus

abstract

  • The BG505 SOSIP.664 trimer-expressing cell lines yield proteins of an appropriate quality for structural studies and animal immunogenicity experiments. The methodology is suitable for making similar lines under Good Manufacturing Practice conditions, to produce trimers for human clinical trials. Moreover, any env gene can be incorporated into this vector system, allowing the manufacture of SOSIP trimers from multiple genotypes, either by transient transfection or from stable cell lines.

publication date

  • April 25, 2014

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC4032163

Digital Object Identifier (DOI)

  • 10.1186/1742-4690-11-33

PubMed ID

  • 24767177

Additional Document Info

start page

  • 33

volume

  • 11

number

  • 1