Multidimensional single-cell analysis of BCR signaling reveals proximal activation defect as a hallmark of chronic lymphocytic leukemia B cells
Gene Expression Regulation, Leukemic
Intracellular Signaling Peptides and Proteins
Leukemia, Lymphocytic, Chronic, B-Cell
Phospholipase C gamma
Receptors, Antigen, B-Cell
Single-cell multidimensional analysis of BCR signaling permitted focused analysis of the variability and heterogeneity of signaling behavior from patient-to-patient, and from cell-to-cell. Disruption of the pSYK/pPLCγ2 relationship is uncovered as a robust hallmark of CLL B cell signaling behavior. Together, these observations implicate novel elements of the BCR signal transduction as potential therapeutic targets.