BRD7, a Tumor Suppressor, Interacts with p85α and Regulates PI3K Activity Academic Article uri icon

Overview

MeSH Major

  • Chromosomal Proteins, Non-Histone
  • Class Ia Phosphatidylinositol 3-Kinase
  • Phosphatidylinositol 3-Kinases
  • Tumor Suppressor Proteins

abstract

  • Phosphoinositide 3-kinase (PI3K) activity is important for regulating cell growth, survival, and motility. We report here the identification of bromodomain-containing protein 7 (BRD7) as a p85α-interacting protein that negatively regulates PI3K signaling. BRD7 binds to the inter-SH2 (iSH2) domain of p85 through an evolutionarily conserved region located at the C terminus of BRD7. Via this interaction, BRD7 facilitates nuclear translocation of p85α. The BRD7-dependent depletion of p85 from the cytosol impairs formation of p85/p110 complexes in the cytosol, leading to a decrease in p110 proteins and in PI3K pathway signaling. In contrast, silencing of endogenous BRD7 expression by RNAi increases the steady-state level of p110 proteins and enhances Akt phosphorylation after stimulation. These data suggest that BRD7 and p110 compete for the interaction to p85. The unbound p110 protein is unstable, leading to the attenuation of PI3K activity, which suggests how BRD7 could function as a tumor suppressor.

publication date

  • April 10, 2014

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC4004185

Digital Object Identifier (DOI)

  • 10.1016/j.molcel.2014.02.016

PubMed ID

  • 24657164

Additional Document Info

start page

  • 193

end page

  • 202

volume

  • 54

number

  • 1