B-RAF kinase drives developmental axon growth and promotes axon regeneration in the injured mature CNS Academic Article uri icon

Overview

MeSH Major

  • Axons
  • Central Nervous System
  • Nerve Regeneration
  • Proto-Oncogene Proteins B-raf
  • Signal Transduction

abstract

  • Activation of intrinsic growth programs that promote developmental axon growth may also facilitate axon regeneration in injured adult neurons. Here, we demonstrate that conditional activation of B-RAF kinase alone in mouse embryonic neurons is sufficient to drive the growth of long-range peripheral sensory axon projections in vivo in the absence of upstream neurotrophin signaling. We further show that activated B-RAF signaling enables robust regenerative growth of sensory axons into the spinal cord after a dorsal root crush as well as substantial axon regrowth in the crush-lesioned optic nerve. Finally, the combination of B-RAF gain-of-function and PTEN loss-of-function promotes optic nerve axon extension beyond what would be predicted for a simple additive effect. We conclude that cell-intrinsic RAF signaling is a crucial pathway promoting developmental and regenerative axon growth in the peripheral and central nervous systems.

publication date

  • January 2014

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC4010899

Digital Object Identifier (DOI)

  • 10.1084/jem.20131780

PubMed ID

  • 24733831

Additional Document Info

start page

  • 801

end page

  • 14

volume

  • 211

number

  • 5