"Ciliophagy": The consumption of cilia components by autophagy. Review uri icon

Overview

MeSH

  • Animals
  • Humans
  • Microtubule-Associated Proteins

MeSH Major

  • Autophagy
  • Cilia
  • Lung
  • Pulmonary Disease, Chronic Obstructive
  • Smoking

abstract

  • Chronic obstructive pulmonary disease (COPD) involves aberrant airway inflammatory responses to cigarette smoke (CS) associated with respiratory epithelial cell cilia shortening and impaired mucociliary clearance (MCC). The underlying cellular and molecular mechanisms for CS-associated cilia shortening have remained incompletely understood. We have previously demonstrated increased autophagy in the lungs of COPD patients; however, whether or not this process is selective for specific autophagic targets in the lung was not elucidated. Based on observations that increased morphological and biochemical indicators of autophagy correlate with cilia shortening in our models, we posited that autophagy might regulate cilia length in response to CS in the lung. We demonstrate that CS-induced cilia shortening occurs through an autophagy-dependent mechanism mediated by the deacetylase HDAC6 (histone deacetylase 6). Autophagy-impaired (Becn1(+/-), map1lc3b(-/-), or Hdac6(-/Y)) mice resist CS-induced cilia shortening. Furthermore, cilia components are identified as autophagic substrates during CS exposure. Assessment of airway cilia function using a 3D MCC assay demonstrates that Becn1(+/-), map1lc3b(-/-), and Hdac6(-/Y) mice or mice injected with the HDAC6 inhibitor tubastatin A are protected from CS-associated mucociliary dysfunction. We concluded that an autophagy-dependent pathway regulates cilia length during CS exposure, which identifies new pathways and targets in COPD.

publication date

  • March 2014

has subject area

  • Animals
  • Autophagy
  • Cilia
  • Humans
  • Lung
  • Microtubule-Associated Proteins
  • Pulmonary Disease, Chronic Obstructive
  • Smoking

Research

keywords

  • Journal Article
  • Review

Identity

Language

  • eng

PubMed Central ID

  • PMC4077895

Digital Object Identifier (DOI)

  • 10.4161/auto.27641

PubMed ID

  • 24401596

Additional Document Info

start page

  • 532

end page

  • 534

volume

  • 10

number

  • 3