Block-Cell-Printing for live single-cell printing Academic Article uri icon

Overview

MeSH Major

  • Cell Engineering
  • Cells
  • Molecular Conformation
  • Tissue Array Analysis

abstract

  • A unique live-cell printing technique, termed "Block-Cell-Printing" (BloC-Printing), allows for convenient, precise, multiplexed, and high-throughput printing of functional single-cell arrays. Adapted from woodblock printing techniques, the approach employs microfluidic arrays of hook-shaped traps to hold cells at designated positions and directly transfer the anchored cells onto various substrates. BloC-Printing has a minimum turnaround time of 0.5 h, a maximum resolution of 5 ┬Ám, close to 100% cell viability, the ability to handle multiple cell types, and efficiently construct protrusion-connected single-cell arrays. The approach enables the large-scale formation of heterotypic cell pairs with controlled morphology and allows for material transport through gap junction intercellular communication. When six types of breast cancer cells are allowed to extend membrane protrusions in the BloC-Printing device for 3 h, multiple biophysical characteristics of cells--including the protrusion percentage, extension rate, and cell length--are easily quantified and found to correlate well with their migration levels. In light of this discovery, BloC-Printing may serve as a rapid and high-throughput cell protrusion characterization tool to measure the invasion and migration capability of cancer cells. Furthermore, primary neurons are also compatible with BloC-Printing.

publication date

  • February 25, 2014

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3939871

Digital Object Identifier (DOI)

  • 10.1073/pnas.1313661111

PubMed ID

  • 24516129

Additional Document Info

start page

  • 2948

end page

  • 53

volume

  • 111

number

  • 8