Flow cytometric differentiation of abnormal and normal plasma cells in the bone marrow in patients with multiple myeloma and its precursor diseases Academic Article uri icon

Overview

MeSH Major

  • Bone Marrow
  • Monoclonal Gammopathy of Undetermined Significance
  • Multiple Myeloma
  • Paraproteinemias
  • Plasma Cells

abstract

  • Flow cytometric (FC) enumeration of abnormal plasma cells (APCs) for diagnosis and prognostication of plasma cell dyscrasias (PCD) is challenging. We studied antigen expression in normal plasma cells (NPC) (N = 34) and APC in a series of unselected PCD (N = 59). NPC subpopulations often demonstrated CD19(-), CD20(+), CD45(-) or dim and CD56(+), an immunophenotype observed in PCD. However abnormal CD81 was only observed in APCs (APC detection sensitivity 95%; specificity 100%). We evaluated differences in antigen expression patterns among MGUS (N = 14), SMM (N = 35) and MM (N = 10), finding the combination of CD45 and CD56 helpful in differentiating MGUS from SMM and MM (p = 0.0002).

publication date

  • March 2014

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3947595

Digital Object Identifier (DOI)

  • 10.1016/j.leukres.2013.12.007

PubMed ID

  • 24462038

Additional Document Info

start page

  • 371

end page

  • 6

volume

  • 38

number

  • 3