Cryo-EM structure of a fully glycosylated soluble cleaved HIV-1 envelope trimer Academic Article uri icon

Overview

MeSH Major

  • Antigens, CD4
  • Models, Molecular
  • env Gene Products, Human Immunodeficiency Virus

abstract

  • The HIV-1 envelope glycoprotein (Env) trimer contains the receptor binding sites and membrane fusion machinery that introduce the viral genome into the host cell. As the only target for broadly neutralizing antibodies (bnAbs), Env is a focus for rational vaccine design. We present a cryo-electron microscopy reconstruction and structural model of a cleaved, soluble Env trimer (termed BG505 SOSIP.664 gp140) in complex with a CD4 binding site (CD4bs) bnAb, PGV04, at 5.8 angstrom resolution. The structure reveals the spatial arrangement of Env components, including the V1/V2, V3, HR1, and HR2 domains, as well as shielding glycans. The structure also provides insights into trimer assembly, gp120-gp41 interactions, and the CD4bs epitope cluster for bnAbs, which covers a more extensive area and defines a more complex site of vulnerability than previously described.

publication date

  • January 2013

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3954647

Digital Object Identifier (DOI)

  • 10.1126/science.1245627

PubMed ID

  • 24179160

Additional Document Info

start page

  • 1484

end page

  • 90

volume

  • 342

number

  • 6165