Dna methylation profiling in human b cells reveals immune regulatory elements and epigenetic plasticity at alu elements during b-cell activation Academic Article uri icon

Overview

MeSH Major

  • Alu Elements
  • B-Lymphocytes
  • DNA Methylation
  • Lymphocyte Activation
  • Regulatory Elements, Transcriptional

abstract

  • Memory is a hallmark of adaptive immunity, wherein lymphocytes mount a superior response to a previously encountered antigen. It has been speculated that epigenetic alterations in memory lymphocytes contribute to their functional distinction from their naive counterparts. However, the nature and extent of epigenetic alterations in memory compartments remain poorly characterized. Here we profile the DNA methylome and the transcriptome of B-lymphocyte subsets representing stages of the humoral immune response before and after antigen exposure in vivo from multiple humans. A significant percentage of activation-induced losses of DNA methylation mapped to transcription factor binding sites. An additional class of demethylated loci mapped to Alu elements across the genome and accompanied repression of DNA methyltransferase 3A. The activation-dependent DNA methylation changes were largely retained in the progeny of activated B cells, generating a similar epigenetic signature in downstream memory B cells and plasma cells with distinct transcriptional programs. These findings provide insights into the methylation dynamics of the genome during cellular differentiation in an immune response.

publication date

  • December 2013

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC3847773

Digital Object Identifier (DOI)

  • 10.1101/gr.155473.113

PubMed ID

  • 24013550

Additional Document Info

start page

  • 2030

end page

  • 41

volume

  • 23

number

  • 12