The Hippo signaling pathway interactome Academic Article uri icon


MeSH Major

  • Drosophila Proteins
  • Drosophila melanogaster
  • Intracellular Signaling Peptides and Proteins
  • Protein Interaction Maps
  • Protein-Serine-Threonine Kinases


  • The Hippo pathway controls metazoan organ growth by regulating cell proliferation and apoptosis. Many components have been identified, but our knowledge of the composition and structure of this pathway is still incomplete. Using existing pathway components as baits, we generated by mass spectrometry a high-confidence Drosophila Hippo protein-protein interaction network (Hippo-PPIN) consisting of 153 proteins and 204 interactions. Depletion of 67% of the proteins by RNA interference regulated the transcriptional coactivator Yorkie (Yki) either positively or negatively. We selected for further characterization a new member of the alpha-arrestin family, Leash, and show that it promotes degradation of Yki through the lysosomal pathway. Given the importance of the Hippo pathway in tumor development, the Hippo-PPIN will contribute to our understanding of this network in both normal growth and cancer.

publication date

  • January 2013



  • Academic Article



  • eng

PubMed Central ID

  • PMC3951131

Digital Object Identifier (DOI)

  • 10.1126/science.1243971

PubMed ID

  • 24114784

Additional Document Info

start page

  • 737

end page

  • 40


  • 342


  • 6159